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Image Search Results
Journal:
Article Title: Significance of Immunological Detection of Human Telomerase Reverse Transcriptase
doi:
Figure Lengend Snippet: A: Telomerase activity in nuclear and cytoplasmic extracts from each cell line. B: Assay to examine whether composition of lysis buffers affect levels of the TRAP activity. Buffer A and C mean lysis buffers with which cytoplasmic and nuclear extracts were extracted, respectively. Nuclear extracts of C33A were diluted with an equal amounts of cytoplasmic lysis buffer (buffer A), and cytoplasmic extracts of C33A were diluted with an equal amounts of nuclear lysis buffer (buffer C). This manipulation equalizes the composition of each extraction buffer. IC, internal control to verify amplification efficiency of PCR.
Article Snippet: TRAP Assay TRAP assays were performed using the
Techniques: Activity Assay, Lysis, Extraction, Control, Amplification
Journal:
Article Title: Significance of Immunological Detection of Human Telomerase Reverse Transcriptase
doi:
Figure Lengend Snippet: Western blot analysis of hTERT expression in cell lines. Nuclear or cytoplasmic extracts (100 μg) from each cell line were electrophoresed on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to membranes, and probed with specific antibodies against hTERT (KM2604) or Sp1. Immunoreactive bands were visualized by the ECL detection system. Specific signals of the expected size (127 kd) were observed in both nucleic and cytoplasmic extracts from cancer cell lines. In contrast, this signal was faint or absent in telomerase-negative SaOs2, SUSUMU-1, HRCEs, and NHFs; additional bands of lower molecular weight (∼110 kd) were observed in these cells. N, nuclear extract; C, cytoplasmic extract.
Article Snippet: TRAP Assay TRAP assays were performed using the
Techniques: Western Blot, Expressing, Polyacrylamide Gel Electrophoresis, Molecular Weight